Tag Chip It Kit

Tag-ChIP-IT Kit addresses a commonly encountered issue when considering ChIP, the lack of available ChIP-grade antibodies to a target of interest. A common solution to this issue is to use an epitope tag, such as FLAG, HA, and GFP, to tag the protein of interest. However, since these tags are not specifically

The Tag-ChIP-IT Kit provides a method to perform ChIP without the need for protein-specific antibodies. Simply create an expression construct containing your protein of interest in-frame with a C-terminal AM-tag, which has been designed to produce highly consistent and reliable ChIP results. The method is ideal when working with targets that

Figure 1 Flow Chart of the Tag-ChIP-IT Assay. Prior to using the Tag-ChIP-IT Kit, the protein of interest is cloned into an expression vector containing the C-terminal AM-tag, such as Active Motif's pAM_1C Empty Vector Catalog No. 53023. Following sequence verification of the cloned insert, cells are transfected for expression of the AM-tagged fusion protein.

Active Motif's Tag-ChIP-IT Kit offers a method to perform ChIP without the need for protein-specific antibodies. Simply create an expression construct containing the protein of interest in-frame with a C-terminal AM-tag that is specifically designed to work in ChIP and produce highly consistent and reliable results.

,bgtActive Motif's Tag-ChIP-IT Kit is designed to enable chromatin immunoprecipitation of transcription factors, sequence variants and truncated proteins that have been expressed using Active Motif's unique AM-tag. The AM-tag is specifically designed to minimize cross-reactivity with mammalian samples, thereby reducing background signal.

Active Motif's Tag-ChIP-IT Kit offers a method to perform ChIP without the need for protein-specific antibodies. Simply create an expression construct containing the protein of interest in-frame with a C-terminal AM-tag that is specifically designed to work in ChIP and produce highly consistent and reliable results.

The Tag-ChIP-IT Kit offers a method to perform chromatin immunoprecipitation ChIP on transcription factor sequence variants, mutations and truncations without the need for protein-specific antibodies. Simply create an expression construct containing the protein of interest in-frame with a C-terminal AM-tag. Active Motif's unique AM-tag sequence is specifically designed to work in ChIP.

The AM-Tag antibody is specifically designed for use with Active Motif's unique AM-Tag sequence in combination with the Tag-ChIP-IT Kit Catalog No. 53022. Simply clone your protein of interest into Active Motif's pAM_1C Empty Vector Catalog No. 53023, or add the AM-Tag sequence to the C-terminus of your protein expression vector.

Figure 1 CUTampTag-IT Assay Kit data correlate well with ENCODE ChIP-Seq data. Chromatin landscapes from human K562 cells are shown across a 868 kb segment of the human genome region Chr16 10,400-11,250 for H3K27me3 dark blue, phospho-Pol2Ser2 red, CTCF orange, H3K4me3 green, H3K9ac light blue H3K9me3 purple, and H3K27ac black

Figure 1 Flow Chart of the Tag-ChIP-IT Assay. Prior to using the Tag-ChIP-IT Kit, the protein of interest is cloned into an expression vector containing the C-terminal AM-tag, such as Active Motif's pAM_1C Empty Vector Catalog No. 53023. Following sequence verification of the cloned insert, cells are transfected for expression of the AM-tagged fusion protein.